Journal: Experimental and therapeutic medicine

Article Title: PHLDA3 inhibition attenuates endoplasmic reticulum stress-induced apoptosis in myocardial hypoxia/reoxygenation injury by activating the PI3K/AKT signaling pathway.

doi: 10.3892/etm.2021.10045

Figure Lengend Snippet: Figure 4. Blockage of the p‑PI3K/AKT pathway inhibits the protective effects of PHLDA3 inhibition following H/R injury. (A) Cell Counting Kit‑8 assays were used to evaluate cell viability. (B) LDH release was detected by ELISA. (C) Apoptotic rate in each group. mRNA levels of ERS markers, including (D) CHOP, (E) GRP78 and (F) caspase‑12, were measured by reverse transcription‑quantitative PCR. Data are presented as the mean ± standard deviation. n=4/group. *P<0.05 vs. H/R + AdshPHLDA3 + PBS. #P<0.05 vs. H/R + AdshRNA + PBS. PHLDA3, pleckstrin homology‑like domain family A member 3; H/R, hypoxia/ reoxygenation; LDF, lactate dehydrogenase; GRP78, 78 kDa glucose‑regulated protein; AdshRNA, adenovirus encoding scrambled short hairpin RNA; AdshPHLDA3, adenoviral vectors encoding PHLDA3 shRNA; NS, not significant.

Article Snippet: Antibodies against p‐AKT (cat. no. 4060; 1:600), AKT (cat. no. 4691; 1:400) and CHOP (cat. no. 5554; 1:600) were purchased from Cell Signaling Technology, Inc. Horseradish peroxidase‐conjugated goat‐anti rabbit secondary antibodies were obtained from BIOSS.

Techniques: Inhibition, CCK-8 Assay, Enzyme-linked Immunosorbent Assay, Standard Deviation, shRNA